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monoclonal antipax7 antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank monoclonal antipax7 antibody
    Monoclonal Antipax7 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antipax7+antibody/anti-Pax7/pm41411128-250-20-23
    Average 99 stars, based on 279 article reviews
    monoclonal antipax7 antibody - by Bioz Stars, 2026-10
    99/100 stars

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    Incubation:

    Article Title: Hindlimb suspension in Wistar rats: Sex-based differences in muscle response.
    Article Snippet: .. Briefly, slides were deparaffinized and rehydrated followed by epitope retrieval using sodium citrate (10 mM, pH 6.5) at 92°C for 20 min. Endogenous peroxidases were blocked with 3% hydrogen peroxide in PBS, and then slides were incubated overnight at 4°C in anti- wheat germ agglutinin conjugated to AF488 tyramide (1:50) (no. W11261; Invitrogen) and antiPax7 antibody (1:100) (Developmental Studies Hybridoma Bank). .. The next day, slides were incubated for 70 min at room temperature in goat anti- mouse biotinylated secondary antibody (no. 115- 065- 205; Jackson ImmunoResearch), and then reacted with streptavidin- horseradish peroxidase (HRP) and AF555 tyramide (1:250) included with the tyramide signal amplification (TSA) kit (#B40933; Invitrogen).

    Article Title: Heterogeneity of Satellite Cells Implicates DELTA1/NOTCH2 Signaling in Self-Renewal.
    Article Snippet: To multiplex PAX7 immunohistochemistry with RNAscope, RNAscope protocol was first performed until detection step, followed by Avidin/Biotin Blocking Kit (Vector Labs SP-2001), Mouse onMouse (M.O.M.) .. Blocking reagent (Vector Labs #MKB-2202), 1:5 antiPAX7 antibody (DSHB, PAX7 supernatant) incubation overnight at 4 C, 1:200 dilution of anti-mouse biotin secondary (provided with Mouse onMouse kit) for 20 minutes at room temperature (RT), 1:5000 dilution of Streptavidin-HRP (Abcam #ab7403) for 5 minutes at e3 Cell Reports 30, 1491–1503.e1–e6, February 4, 2020 RT, 30 minute 0.3% Triton X-100 in PBS wash, and detected with Peroxidase Substrate Kit DAB (Vector Labs #SK-4100) for 20 minutes. .. Other antibodies used were anti-NOTCH2 (Cell Signaling, D67A6, 1:100 dilution), anti-SPP1 (R&D Systems, AF808, dilution 1:1000), anti-tdTomato (Biorbyt, orb182397, dilution 1:250).

    Blocking Assay:

    Article Title: Heterogeneity of Satellite Cells Implicates DELTA1/NOTCH2 Signaling in Self-Renewal.
    Article Snippet: To multiplex PAX7 immunohistochemistry with RNAscope, RNAscope protocol was first performed until detection step, followed by Avidin/Biotin Blocking Kit (Vector Labs SP-2001), Mouse onMouse (M.O.M.) .. Blocking reagent (Vector Labs #MKB-2202), 1:5 antiPAX7 antibody (DSHB, PAX7 supernatant) incubation overnight at 4 C, 1:200 dilution of anti-mouse biotin secondary (provided with Mouse onMouse kit) for 20 minutes at room temperature (RT), 1:5000 dilution of Streptavidin-HRP (Abcam #ab7403) for 5 minutes at e3 Cell Reports 30, 1491–1503.e1–e6, February 4, 2020 RT, 30 minute 0.3% Triton X-100 in PBS wash, and detected with Peroxidase Substrate Kit DAB (Vector Labs #SK-4100) for 20 minutes. .. Other antibodies used were anti-NOTCH2 (Cell Signaling, D67A6, 1:100 dilution), anti-SPP1 (R&D Systems, AF808, dilution 1:1000), anti-tdTomato (Biorbyt, orb182397, dilution 1:250).



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